Catalog #: AP14138 <br>Name: Biotin-HPDP <br>Chemical Formula: C24H37N5O3S3 <br>Molecular Weight: 539.8 <br>CAS: 129179-83-5 <br>Purity: ≥95% <br>Availability: In Stock <br> <br>DESCRIPTION <br>Biotin HPDP CAS:129179-83-5 is a thiol-reactive biotinylating reagent that is a membrane-permeable biotin-labeling reagent that forms reversible disulfide bonds. It is used to label protein cysteine and other thiol-containing substrates. The disulfide bond created between the target sulfhydryl molecule and the biotin group can be cleaved by a reducing agent to release the biotin group and regenerate the protein (or peptide) in its original unmodified form. The interaction of biotin-HPDP with thiol-containing substrates is readily carried out in buffers such as PBS at pH 6.5 to 7.5 by cleavage of disulfide bonds with dithiothreitol (DTT) or other reducing agents, whereas Rather than attempting to dissociate the high affinity interaction between avidin and biotin with strong acids or denaturing agents, the captured biotinylated molecules can be efficiently eluted from the support. <br> <br>AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis. <br> <br>SYNONYMS <br>Biotin-HPDP <br>129179-83-5 <br>n-(6-(Biotinamido)hexyl)-3-(2-pyridyldithio)propionamide <br>N-[6-(Biotinamido)hexyl]-3-(2-pyridyldithio)propionamide <br>5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)-N-(6-(3-(pyridin-2-yldisulfanyl)propanamido)hexyl)pentanamide <br>BiotinHPDP <br>Pyridyldithiol-biotin <br>Pyridyldisulfide-biotin <br>5-[(3aS,4S,6aR)-2-oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]-N-[6-[3-(pyridin-2-yldisulfanyl)propanoylamino]hexyl]pentanamide <br>N-[6-(biotinamido)hexyl]-3-(2-pyridyldithio)propionamide <br>N-[6-(Biotinamido)hexyl]-3-(2-pyridyldithio)-propionamide <br>N-[6-(Biotinamido)hexyl]-3a?(2a?pyridyldithio)-propionamide <br>(3aS,3abeta,6abeta)-N-[6-[3-(2-Pyridyldithio)propionylamino]hexyl]hexahydro-2-oxo-1H-thieno[3,4-d]imidazole-4alpha-pentanamide <br>5-[(3aS,4S,6aR)-2-Oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl]-N-(6-{3-[(pyridin-2-yl)disulfanyl]propanamido}hexyl)pentanamide <br> <br>SHIPPING &amp; DELIVERY <br>Ships within: 24 hours <br>Storage condition: -20 ° C <br>Shipping: Ambient Temperature <br> <br>For more details of bioanalysis services, please contact us. <br>#biotin#hpdp <br>[a]https%3A%2F%2Faxispharm.com%2Fproduct%2Fbiotin-hpdp%2F[/a]

Catalog #: AP10507 <br>Name: Biotin amine <br>Chemical Formula: C12H22N4O2S <br>Molecular Weight: 286.4 <br>CAS: 1231488-78-0,111790-37-5 <br>Purity: ≥95% <br>Availability: In Stock <br>SDS: Download <br> <br>DESCRIPTION <br>Axispharm offers high-purity Biotin-EDA, a simple biotinylation of antibodies, proteins and any other carboxyl-containing biomolecules. The primary amine group of this biotin amine reagent can react with -NHS, -COOH and other moieties to form stable and irreversible amide bonds. <br> <br>Axis Pharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis. <br> <br>SYNONYMS <br>N-(2-Aminoethyl)biotinamide <br>111790-37-5 <br>Biotin-EDA <br>N-(2-Aminoethyl)-5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamide <br>(+)-Biotin-amine <br>Biotinylamidoethylacetamide <br>5-[(3aS,4S,6aR)-2-oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]-N-(2-aminoethyl)pentanamide <br>BAEA <br>biotinylethylenediamine <br>D-Biotin-EDA <br>(+)-Biotin-NH2 <br>(+)-Biotinyl ethylamine <br>N-(2-aminoethyl) biotinamide <br>1H-Thieno(3,4-d)imidazole-4-pentanamide, N-(2-aminoethyl)hexahydro-3-oxo-, (3aS,4S,6aR)- N-(2-Aminoethyl)-5-(2-hydroxy-3a,4,6,6a-tetrahydro-1H-thieno[3,4-d]imidazol-6-yl)pentanimidic ac <br> <br>SHIPPING &amp; DELIVERY <br>Ships within: 24 hours <br>Storage condition: -20 ° C <br>Shipping: Ambient Temperature <br> <br>For more details of bioanalytical services, please contact us. <br>#biotin#amine <br>[a]https%3A%2F%2Faxispharm.com%2Fproduct%2Fbiotin-amine%2F[/a]

Catalog #: AP14418 <br>Name: BDP FL-PEG4-amine TFA salt <br>Chemical Formula: C24H37BF2N4O5 <br>Molecular Weight: 510.4 <br>CAS: 2183473-14-3 <br>Purity: ≥95% <br>Availability: In Stock <br> <br>DESCRIPTION <br>BDP Bodipy FL-PEG4-amine TFA salt CAS:2183473-14-3 is a fluorescent PEG derivative containing an amine group and a hydrophilic PEG spacer arm. The amine group makes the compound more reactive toward carboxylic acids, NHS esters, and other carbonyl groups. PEG linkers and derivatives can be used to develop antibody drug conjugates. <br> <br>AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis. <br> <br>SYNONYMS <br>BDP FL-PEG4-amine TFA salt <br>BDP FL-PEG4-amine <br>2183473-14-3 <br>BDP FL-PEG4-amine TFA <br>N-[2-[2-[2-[2-(2-aminoethoxy)ethoxy]ethoxy]ethoxy]ethyl]-3-(2,2-difluoro-10,12-dimethyl-3-aza-1-azonia-2-boranuidatricyclo[7.3.0.03,7]dodeca-1(12),4,6,8,10-pentaen-4-yl)propanamide <br> <br>SHIPPING &amp; DELIVERY <br>Ships within: 24 hours <br>Storage condition: -20 ° C <br>Shipping: Ambient Temperature <br> <br>For more details of proteomics services, please contact us. <br> <br>As a contract research organization san diego, we will do our best to meet all the needs of clients. <br>#tfa#salt <br>[a]https%3A%2F%2Faxispharm.com%2Fproduct%2Fbdp-fl-peg4-amine-tfa-salt%2F[/a]

Catalog #: AP10233 <br>Name: BCN-OH <br>Chemical Formula: C10H14O <br>Molecular Weight: 150.22 <br>CAS: 1379662-52-8 <br>Purity: ≥95% <br>Availability: In Stock <br>SDS: Download <br> <br>DESCRIPTION <br>Cyclooctynes/BCN OH are useful in strain-promoted copper-free click chemistry cycloaddition reactions with 1,2,4,5-tetrazines. The cycloaddition between cyclooctyne and tetrazines is the fastest biologically compatible ligation technology reported. It has had many reported applications in biological labeling and imaging. <br> <br>Axis Pharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis. <br> <br>SYNONYMS <br>bicyclo[6.1.0]non-4-yn-9-ylmethanol <br>endo BCN-OH <br>9-bicyclo[6.1.0]non-4-ynylmethanol <br>(1R,8S,9s)-Bicyclo[6.1.0]non-4-yn-9-ylmethanol <br>Bicyclo[6.1.0]nona-4-yne-9-methanol <br> <br>SHIPPING &amp; DELIVERY <br>Ships within: 24 hours <br>Storage condition: -20 ° C <br>Shipping: Ambient Temperature <br> <br>We can also offer high quality adc linker for clients, if you have needs, please contact us. <br>#bcn#oh <br>[a]https%3A%2F%2Faxispharm.com%2Fproduct%2Fbcn-oh%2F[/a]

Catalog #: AP12398 <br>Name: BCN-C3-Trimethoxy Silane <br>Chemical Formula: C17H29NO5Si <br>Molecular Weight: 355.51 <br>CAS: N/A <br>Purity: ≥95% <br>Availability: In Stock <br> <br>DESCRIPTION <br>BCN-C3-Trimethoxy Silane is a chemical compound that contains several functional groups, including a vinyl group, a trimethoxy silane group, and a bicyclo[6.1.0]nonyne group. This compound is widely used in the field of organic synthesis, particularly in bioconjugation and drug delivery. <br> <br>The vinyl group in BCN-C3-Trimethoxy Silane enables it to undergo various types of chemical reactions, including addition, elimination, and polymerization reactions. The trimethoxy silane group, on the other hand, can be used as a protective group for alcohols, amines, and carboxylic acids, or as a linker for attaching other functional groups to the compound. The bicyclo[6.1.0]nonyne group is an extremely reactive strain-promoted alkyne that can undergo rapid and selective reactions with azides, enabling the formation of triazole linkages. <br> <br>AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis. <br> <br>SHIPPING &amp; DELIVERY <br>Ships within: 24 hours <br>Storage condition: -20 ° C <br>Shipping: Ambient Temperature <br> <br>If you want to know more about the ultimate guide to fluorescent dye, please visit our website. <br>#trimethoxy#silane <br>[a]https%3A%2F%2Faxispharm.com%2Fproduct%2Fbcn-c3-trimethoxy-silane%2F[/a]

Catalog #: AP12951 <br>Name: Azido-PEG4-alpha-D-mannose <br>Chemical Formula: C14H27N3O9 <br>Molecular Weight: 381.38 <br>CAS: 1632372-86-1 <br>Purity: ≥95% <br>Availability: In Stock <br> <br>DESCRIPTION <br>Azido PEG4 alpha D mannose CAS:1632372-86-1 is a small molecule linker that contains several functional groups. It has an azido group, which is a reactive functional group that can participate in copper-catalyzed click chemistry reactions, and a PEG4 (polyethylene glycol) chain, which can improve the solubility, stability, and biocompatibility of the compound. Additionally, it has an alpha-D-mannose group, which can be used to target specific cells or tissues that express mannose-binding proteins. The combination of these functional groups makes Azido-PEG4-alpha-D-mannose a versatile tool for bioconjugation and drug delivery applications, particularly in the development of targeted therapeutics for diseases such as cancer and inflammation. <br> <br>Axis Pharma offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis. <br> <br>SYNONYMS <br>(2S,3S,4S,5S,6R)-2-[2-[2-[2-(2-azidoethoxy)ethoxy]ethoxy]ethoxy]-6-(hydroxymethyl)oxane-3,4,5-triol <br>Azido-PEG4-alpha-D- mannose <br> <br>SHIPPING &amp; DELIVERY <br>Ships within: 24 hours <br>Storage condition: -20 ° C <br>Shipping: Ambient Temperature <br> <br>If you want to know more about antibody drug conjugate linkers, please visit our website. <br>#alpha#d#mannose <br>[a]https%3A%2F%2Faxispharm.com%2Fproduct%2Fazido-peg4-alpha-d-mannose%2F[/a]

Catalog #: AP11158 <br>Name: Azidoethyl-SS-ethylamine <br>Chemical Formula: C4H10N4S2 <br>Molecular Weight: 178.27 <br>CAS: 1807512-40-8 <br>Purity: ≥95% <br>Availability: In Stock <br>SDS: Download <br> <br>DESCRIPTION <br>Azidoethyl SS ethylamine CAS:1807512-40-8 is a chemical compound that contains two important functional groups: an azide (-N3) and a thiol (-SH) group. The azide group makes the compound highly reactive and suitable for use in a variety of chemical reactions, while the thiol group is commonly used in bioconjugation and protein modification studies. Azidoethyl-SS-ethylamine is often used as a crosslinking agent in chemical biology and bioconjugation applications due to its ability to selectively label and modify proteins and other biological molecules. <br> <br>As a contract research organization san diego, AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis. <br> <br>SYNONYMS <br>Azido-cystamine hydrochloride <br>2-((2-azidoethyl)disulfanyl)ethan-1-amine <br>2-(2-azidoethyldisulfanyl)ethanamine <br> <br>SHIPPING &amp; DELIVERY <br>Ships within: 24 hours <br>Storage condition: -20 ° C <br>Shipping: Ambient Temperature <br> <br>If you want to know more about bioanalysis services, please visit our website. <br>#ethylamine <br>[a]https%3A%2F%2Faxispharm.com%2Fproduct%2Fazidoethyl-ss-ethylamine%2F[/a]

Catalog #: AP14307 <br>Name: ARV-771 <br>Chemical Formula: C49H60ClN9O7S2 <br>Molecular Weight: 986.7 <br>CAS: 1949837-12-0 <br>Purity: ≥95% <br>Availability: In Stock <br> <br>DESCRIPTION <br> <br>ARV 771 is a bromodomain and extraterminal (BET) protein degrader, a novel BET-PROTAC (proteolytic targeting chimera) with a HIF-1-derived von Hippel-Landau (VHL) E3 ligase Conjugated hydroxyproline and BET conjugated triazolodiazepine. <br> <br>AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis. <br> <br>SYNONYMS <br>ARV-771 <br>1949837-12-0 <br>(2S,4R)-1-[(2S)-2-[[2-[3-[2-[[2-[(9S)-7-(4-chlorophenyl)-4,5,13-trimethyl-3-thia-1,8,11,12-tetrazatricyclo[8.3.0.02,6]trideca-2(6),4,7,10,12-pentaen-9-yl]acetyl]amino]ethoxy]propoxy]acetyl]amino]-3,3-dimethylbutanoyl]-4-hydroxy-N-[(1S)-1-[4-(4-methyl-1,3-thiazol-5-yl)phenyl]ethyl]pyrrolidine-2-carboxamide <br>ARV771 <br>(2S,4R)-1-((2S)-2-(tert-butyl)-15-((6S)-4-(4-Chlorophenyl)-2,3,9-trimethyl-6H-thieno[3,2-f][1,2,4]triazolo[4,3-a][1,4]diazepin-6-yl)-4,14-dioxo-6,10-dioxa-3,13-diazapentadecan-1-oyl)-4-hydroxy-N-((S)-1-(4-(4-methylthiazol-5-yl)phenyl)ethyl)pyrrolidine-2-carboxamide <br>(2S,4R)-1-((S)-2-(tert-butyl)-15-((S)-4-(4-chlorophenyl)-2,3,9-trimethyl-6H-thieno[3,2-f][1,2,4]triazolo[4,3-a][1,4]diazepin-6-yl)-4,14-dioxo-6,10-dioxa-3,13-diazapentadecan-1-oyl)-4-hydroxy-N-((S)-1-(4-(4-methylthiazol-5-yl)phenyl)ethyl)pyrrolidine-2-carboxamide <br>(2S,4R)-1-((S)-2-(tert-butyl)-15-((S)-4-(4-chlorophenyl)-2,3,9-trimethyl-6H-thieno[3,2-f][1,2,4]triazolo[4,3-a][1,4]diazepin-6-yl)-4,14-dioxo-6,10-dioxa-3,13-diazapentadecanoyl)-4-hydroxy-N-((S)-1-(4-(4-methylthiazol-5-yl)phenyl)ethyl)pyrrolidine-2-carboxamide <br>(2S,4R)-1-[(2S)-2-[[2-[3-[2-[[2-[(9S)-7-(4-chlorophenyl)-4,5,13-trimethyl-3-thia-1,8,11,12-tetrazatricyclo[8.3.0.02,6]trideca-2(6),4,7,10,12-pentaen-9-yl]acetyl]amino]ethoxy]propoxy]acetyl]amino]-3,3-dimethylbutanoyl]-4-hydroxy-N-[(1S)-1-[4-(4-methyl-1,3- <br>N-({3-[2-({[(6S)-4-(4-Chlorphenyl)-2,3,9-trimethyl-6H-thieno[3,2-f][1,2,4]triazolo[4,3-a][1,4]diazepin-6-yl]acetyl}amino)ethoxy]propoxy}acetyl)-3-methyl-L-valyl-(4R)-4-hydroxy-N-{(1S)-1-[4-(4-methyl-1 ,3-thiazol-5-yl)phenyl]ethyl}-L-prolinamid <br> <br>SHIPPING &amp; DELIVERY <br>Ships within: 24 hours <br>Storage condition: -20 ° C <br>Shipping: Ambient Temperature <br> <br>If you want to know more about bioanalytical services, please visit our website. <br>#arv#771 <br>[a]https%3A%2F%2Faxispharm.com%2Fproduct%2Farv-771%2F[/a]

Catalog #: AP12453 <br>Name: APDye Fluor 647 NHS Ester <br>Chemical Formula: C40H46N3Na3O16S4 <br>Molecular Alexa Fluor 647 Molecular Weight: 1022 <br>CAS: 407627-60-5 <br>Solubility: Water, DMSO, DMF <br>Abs/Em Maxima: 649/671 nm <br>Extinction coefficient: 270,000 cm-1M-1 <br>Spectrally similar dyes: Cy5, DyLight® 649, <br>Purity: ≥95% <br>Availability: In Stock <br> <br>DESCRIPTION <br>APDye Fluor 647 NHS Ester (Alexa Fluor ® 647 analog) CAS:407627-60-5 is a water-soluble, bright, far-red-fluorescent dye with excitation ideally suited for the 633 nm or 647 nm laser lines. A significant advantage to using long wavelength dyes such as Cy5 or APDye™ Fluor 647 dye over other fluorophores is the low autofluorescence of biological specimens in this region of the spectrum. <br>The NHS ester (or succinimidyl ester) is the most popular amine reactive group for labeling with the primary amines of proteins (Lys), amine-modified oligonucleotides, and other amine-containing molecules. Proteins can be labeled with APDye™ Fluor 647 NHS Ester at high molar ratios without significant self-quenching, leading to brighter conjugates and more sensitive detection. APDye™ Fluor 647 conjugates are often a probe of choice for detection of low abundance targets. <br>APDye™ Fluor 647 is structurally similar to Alexa Fluor® 647, and spectrally is almost identical to Cy5 Dye, Alexa Fluor® 647, CF® 647 Dye, or any other Cyanine5 based fluorescent dyes. <br> <br>Axis Pharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis. <br> <br>SHIPPING &amp; DELIVERY <br>Ships within: 24 hours <br>Storage condition: -20 ° C <br>Shipping: Ambient Temperature <br> <br>If you want to know more about proteomics services, please visit our website. <br>#alexa#fluor#647#molecular#weight <br>[a]https%3A%2F%2Faxispharm.com%2Fproduct%2Fapdye-647-nhs-ester%2F[/a]

Catalog #: AP12455 <br>Name: APDye Fluor 647 Maleimide <br>Chemical Formula: C46H57N4Na3O15S4 <br>Molecular Weight: 1103.2 <br>Solubility: Water, DMSO, DMF <br>Abs/Em Maxima: 649/671 nm <br>Extinction coefficient: 270,000 cm-1M-1 <br>Spectrally similar dyes: Cy5, DyLight® 649, <br>Purity: ≥98% <br>Availability: In Stock <br> <br>DESCRIPTION <br>APDye™ alexa fluor 647 maleimide is a water-soluble, far-red-fluorescent dye that is spectrally similar to other 650 dyes that are used for flow cytometry, microscopy and other applications. APDye™ Fluor 647 conjugates ideally suited for the 633 nm or 647 nm laser lines. A significant advantage to using long wavelength dyes such as Cy5 or APDye™ Fluor 647 over other fluorophores is the low autofluorescence of biological specimens in this region of the spectrum. <br>Maleimide is the most popular sulfhydryl-reactive reactive group for conjugating the dye to a thiol group on a protein, oligonucleotide thiophosphate, or low molecular weight ligand. The maleimide group specifically and efficiently reacts with reduced thiols (sulfhydryl groups, –SH) at pH 6.5 to 7.5 to form stable thioether bond. The resulting conjugates exhibit brighter fluorescence and greater photostability than the conjugates of many other spectrally similar fluorophores. <br>APDye™ Fluor 647 is structurally similar to Alexa Fluor® 647, and spectrally is almost identical to Cy5 Dye, Alexa Fluor® 647, CF® 647 Dye, or any other Cyanine5 based fluorescent dyes. <br> <br>Axis Pharma offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis. <br> <br>SHIPPING &amp; DELIVERY <br>Ships within: 24 hours <br>Storage condition: -20 ° C <br>Shipping: Ambient Temperature <br> <br>If you want to know more about adc linker, please visit our website. <br>#alexa#fluor#647#maleimide <br>[a]https%3A%2F%2Faxispharm.com%2Fproduct%2Fapdye-647-maleimide%2F[/a]

[a]https%3A%2F%2Faxispharm.com%2Fthe-ultimate-guide-to-fluorescent-dye%2F[/a] <br>#Fluorescent <br>The Ultimate Guide to Fluorescent Dye  <br>Fluorescent dyes, also known as fluorophores, are compounds that absorb light at one wavelength and emit light at a higher wavelength, resulting in fluorescence. These fluorescent dyes can be categorized into various groups, including organic dyes such as fluorescein, rhodamine, and AMCA, biological fluorophores like green fluorescent protein, phycoerythrin, and allophycocyanin, and quantum dots. They are commonly employed in labeling biomolecules with fluorescence. Notably, they offer advantages of smaller size and greater photostability compared to fluorescent proteins. <br> <br>Furthermore, fluorophores are considered safe for usage and do not necessitate radiological controls. Multiple fluorophores can be utilized simultaneously without interference as long as their emission spectra do not overlap. <br> <br>Fluorescent Dyes Examples by Emission Color <br>Blue/Cyan Emitting Fluorescent Dyes: <br>Hoechst dyes: Emit blue fluorescence and are commonly used for DNA staining in microscopy. <br>DAPI (4′,6-Diamidino-2-Phenylindole): Emit blue fluorescence and are also used for DNA staining. <br>Green Emitting Fluorescent Dyes: <br>FITC (Fluorescein Isothiocyanate): Emit green fluorescence and are widely used in biochemistry and cell biology. <br>GFP (Green Fluorescent Protein): Naturally occurring protein that emits green fluorescence and is used in molecular and cell biology. <br>Alexa Fluor 488: Emit green fluorescence and are popular for conjugation to antibodies for immunofluorescence studies. <br>Yellow/Orange Emitting Fluorescent Dyes: <br>Rhodamine dyes: Emit orange to red fluorescence and are commonly used in microscopy and flow cytometry. <br>Texas Red: Emit red fluorescence and are used in various imaging applications. <br>Cy3 (Cyanine 3): Emit orange-red fluorescence and are used in nucleic acid and protein labeling. <br>Red Emitting Fluorescent Dyes: <br>Cy5 (Cyanine 5): Emit deep red fluorescence and are used in a variety of applications including DNA sequencing and protein labeling. <br>Alexa Fluor 647: Emit red fluorescence and are frequently used in multicolor fluorescence imaging. <br>Propidium Iodide: Emit red fluorescence and are used for cell cycle analysis and DNA staining. <br>Near-Infrared Emitting Fluorescent Dyes: <br>IRDye 800CW: Emit near-infrared fluorescence and are used in applications like in vivo imaging. <br>Cy7 (Cyanine 7): Emit near-infrared fluorescence and are utilized in molecular imaging and flow cytometry. <br> <br>Fluorescent Dyes Applications <br>Cellular Imaging and Microscopy: Fluorescent dyes are extensively used to visualize cellular structures, organelles, and molecules under a microscope. They aid researchers in studying cellular processes, protein localization, and intracellular dynamics. <br> <br>Protein Labeling and Tracking: Fluorescent tags attached to proteins enable real-time tracking of their movement and interactions within cells. This aids in studying protein function and cellular processes. <br> <br>Immunofluorescence Assays: Fluorescent labeled antibodies are employed to detect specific antigens in tissues or cells. This is crucial for understanding disease mechanisms and diagnosing medical.

[a]https%3A%2F%2Faxispharm.com%2Fthe-ultimate-guide-to-fluorescent-dye%2F[/a] <br>#Fluorescent <br>The Ultimate Guide to Fluorescent Dye  <br>Fluorescent dyes, also known as fluorophores, are compounds that absorb light at one wavelength and emit light at a higher wavelength, resulting in fluorescence. These fluorescent dyes can be categorized into various groups, including organic dyes such as fluorescein, rhodamine, and AMCA, biological fluorophores like green fluorescent protein, phycoerythrin, and allophycocyanin, and quantum dots. They are commonly employed in labeling biomolecules with fluorescence. Notably, they offer advantages of smaller size and greater photostability compared to fluorescent proteins. <br> <br>Furthermore, fluorophores are considered safe for usage and do not necessitate radiological controls. Multiple fluorophores can be utilized simultaneously without interference as long as their emission spectra do not overlap. <br> <br>Fluorescent Dyes Examples by Emission Color <br>Blue/Cyan Emitting Fluorescent Dyes: <br>Hoechst dyes: Emit blue fluorescence and are commonly used for DNA staining in microscopy. <br>DAPI (4′,6-Diamidino-2-Phenylindole): Emit blue fluorescence and are also used for DNA staining. <br>Green Emitting Fluorescent Dyes: <br>FITC (Fluorescein Isothiocyanate): Emit green fluorescence and are widely used in biochemistry and cell biology. <br>GFP (Green Fluorescent Protein): Naturally occurring protein that emits green fluorescence and is used in molecular and cell biology. <br>Alexa Fluor 488: Emit green fluorescence and are popular for conjugation to antibodies for immunofluorescence studies. <br>Yellow/Orange Emitting Fluorescent Dyes: <br>Rhodamine dyes: Emit orange to red fluorescence and are commonly used in microscopy and flow cytometry. <br>Texas Red: Emit red fluorescence and are used in various imaging applications. <br>Cy3 (Cyanine 3): Emit orange-red fluorescence and are used in nucleic acid and protein labeling. <br>Red Emitting Fluorescent Dyes: <br>Cy5 (Cyanine 5): Emit deep red fluorescence and are used in a variety of applications including DNA sequencing and protein labeling. <br>Alexa Fluor 647: Emit red fluorescence and are frequently used in multicolor fluorescence imaging. <br>Propidium Iodide: Emit red fluorescence and are used for cell cycle analysis and DNA staining. <br>Near-Infrared Emitting Fluorescent Dyes: <br>IRDye 800CW: Emit near-infrared fluorescence and are used in applications like in vivo imaging. <br>Cy7 (Cyanine 7): Emit near-infrared fluorescence and are utilized in molecular imaging and flow cytometry. <br> <br>Fluorescent Dyes Applications <br>Cellular Imaging and Microscopy: Fluorescent dyes are extensively used to visualize cellular structures, organelles, and molecules under a microscope. They aid researchers in studying cellular processes, protein localization, and intracellular dynamics. <br> <br>Protein Labeling and Tracking: Fluorescent tags attached to proteins enable real-time tracking of their movement and interactions within cells. This aids in studying protein function and cellular processes. <br> <br>Immunofluorescence Assays: Fluorescent labeled antibodies are employed to detect specific antigens in tissues or cells. This is crucial for understanding disease mechanisms and diagnosing medical.