#co2 #chromatography <br>[a]https%3A%2F%2Fwww.jshanbon.com%2Fproducts%2Fsupercritical-fluid-chromatography%2F[/a] <br> <br>SFC (Super Fluid Chromatography) provides precise control over the strength, pressure, and temperature of the mobile phase, enabling fine adjustments to the separation ability and selectivity of the system. This flexibility allows for accurate tuning of the chromatographic conditions to achieve optimal separation and desired analytical outcomes. <br> <br>Chiral Splitting Using SFC (Super Fluid Chromatography) <br>Since the &quot;reaction stop&quot; incident, countries have started to pay attention to the pharmacological activity of isomers in chiral drugs because of the different activities of different isomers. The main chiral dismantling methods are： <br>Crystallization <br>Chemical cleavage <br>Biological dismantling <br> <br>Chromatographic methods, including gas chromatography, for the separation of volatile chiral substances; high performance liquid chromatography (HPLC), which uses a chiral stationary phase with good stability, is an important chiral separation method; supercritical fluid chromatography (SFC), which is environmentally friendly, highly efficient, has a large sample throughput and low cost, has increasingly replaced HPLC as the first choice for chiral separation. <br> <br>The Difference Between SFC and HPLC <br>Preparative high-performance liquid chromatography (Prep HPLC) has been one of the most commonly used purification techniques for over 20 years. Specifically, it is a popular separation process in the fine chemicals, pharmaceutical, and biotechnology industries and is widely used in product purification operations. Over this time, Prep HPLC has developed into a fairly effective and applicable technique, particularly for non-chiral purifications. <br> <br>Although popular, Prep HPLC has several drawbacks. The volume of the mobile phase required to purify a specified mass of the compound is large compared to the total sample volume being processed. A typical Prep HPLC fraction contains a large amount of solvent (organic and aqueous) and because of the time required to dry and collect the final product a major advantage of Prep SFC is that the use of CO2 replaces most of the mobile phase, saving solvent usage. This is a major advantage of Prep SFC, as it uses CO2 to replace most of the mobile phase, saving solvent use and effort. <br> <br>A major advantage of Prep SFC is that it uses CO2 instead of most of the mobile phase, saving solvent usage and making it greener. In SFC, the separation efficiency is better due to the low viscosity and high diffusion rate of the mobile phase, allowing for faster and more efficient chromatographic separations. <br> <br>We provide supercritical fluid chromatography equipment, sfc purification system, sfc mobile phase, supercritical fluid chromatography mass spectrometry, co2 chromatography, supercritical co2 chromatography, mobile phase in supercritical fluid chromatography, etc. For supercritical fluid chromatography makes use of supercritical fluid as or more information, please feel free to contact us!

#single #use #filtration <br>[a]https%3A%2F%2Fwww.jshanbon.com%2Fproducts%2Fsingle-use-tangential-flow-filtration-system%2F[/a] <br> <br>The pump heads, valves, piping and liquid contact parts such as pressure sensors, flow meters, pH, conductivity and UV detectors of single-use UF/DF systems are all made of disposable replaceable parts, all of which are designed to be pre-packaged aseptically in advance and installed directly after unpacking before use, eliminating the need for CIP verification. Particularly suitable for applications where multiple CDMOs or multiple products are in common line. <br> <br>Features of single use tff system <br>01. <br>Direct replacement of any components that come into touch with liquid is quick and easy; <br> <br>02. <br>Give FDA and USP VI certifications for non-stainless steel components coming into contact with liquid; <br> <br>03. <br>Software that is similar to non-disposable systems makes it simple to understand and operate. <br> <br>For more information about single use tangential flow filtration, please feel free to contact us!

#single #use #chromatography <br>[a]https%3A%2F%2Fwww.jshanbon.com%2Fproducts%2Fsingle-use-system%2F[/a] <br> <br>Single use filtration systems specifically designed for the amplification and production of processes in fields such as ADC, mRNA vaccines, plasmid DNA, genes, and cell therapies, utilizing disposable pipelines and flow pools to achieve fast and flexible biological processes. <br> <br>Types of Single-use System <br>Single use systems for biopharmaceuticals <br>Replaceable disposable parts make up the pump heads, valves, tubing, and liquid-contact portions of single-use chromatography systems such pressure transducers, flow meters, pH, conductivity, and UV detectors. <br> <br>Single use filtration System <br>There is no requirement for CIP verification because all parts are made to be aseptically pre-packaged beforehand and inserted right after unpacking before use. especially well-suited to applications where various CDMOs or numerous goods are used in the same line. <br> <br>We provide single use biopharma, single use chromatography, single use technology in biopharmaceutical manufacture, single use biopharma processing, etc. For more information, please feel free to contact us!

#single #use #biopharma <br>[a]https%3A%2F%2Fwww.jshanbon.com%2Fproducts%2Fsingle-use-chromatography-system%2F[/a] <br> <br>The pump heads, valves, tubing and liquid contact components of single use chromatography skid systems such as pressure transducers, flow meters, pH, conductivity and UV detectors are made up of disposable replaceable parts, all of which can be packaged aseptically in advance and installed directly after unpacking prior to use without the need for CIP validation. Suitable for use in virus purification or multiple product co-lines. <br> <br>Features of Single use Chromatography System <br>01. <br>All parts that come into contact with the liquid can be directly replaced, which is fast and convenient; <br> <br>02. <br>Provide FDA and USP VI certificates for non stainless steel materials in contact with the material liquid; <br> <br>03. <br>Using software that is homologous to non disposable systems allows for fast learning and easy use.

Hanbon is a manufacturer of liquid ssmb chromatography series products with a large production scale and a complete range of products in China. <br> <br>The main products include: Biopharm purification equipment, Oligo synthesizer, industrial preparation simulated moving bed smb chromatography systems, supercritical fluid chromatography systems, continuous chromatography systems, laboratory chromatography instruments, etc., and can provide users with integrated solutions and services centered on chromatographic products. <br> <br>Synthesis Principle Of Oligonucleotide <br>Most oligonucleotides are currently synthesized by solid-phase synthesis, using automated equipment, in which phosphoramidite monomers are required. For example, the phosphoramidite monomer for DNA synthesis mainly consists of bases, deoxyribose, 2-cyanoethyl 5&#039;-DMT and 3&#039;-terminus, and diisopropylamine group, while the phosphoramidite monomer for RNA requires protection of 2&#039;-OH to prevent cross-reaction between 2&#039;-OH and 5&#039;-OH in solid-phase synthesis, and the protecting group is usually TBDMS. Solid-phase synthesis is frequently used nowadays, the traditional solid-phase carriers were controlled microporous glass beads (CPG), but the loading capacity was limited, only 70-80 μmol/g. Nowadays, more high loading capacity solid-phase carriers are used, and the loading capacity is generally up to 250umol/g~350umol/g. <br> <br>Purification Method Of Oligonucleotide <br>Oligonucleotides are short-stranded nucleic acids consisting of less than 20 nucleotide units, and purification methods can be selected according to different oligonucleotide lengths, sequences, structures, and purity requirements. The following are some common oligonucleotide purification methods: <br> <br>1. Polyacrylamide gel electrophoresis (PAGE): Suitable for the purification of oligonucleotides with short length (less than 30 nucleotide units). The size difference between oligonucleotides and impurities is separated by PAGE, followed by cleavage and elution of the target oligonucleotides. <br> <br>2. Reversed-phase high-performance liquid chromatography (RP-HPLC): For purification with a length of 10-50 nucleotide units, oligonucleotides are separated from impurities based on polarity differences. <br> <br>3. Ion exchange chromatography (IEX): For purification of 10-100 nucleotide units in length, oligonucleotides and impurities are separated by adsorption and elution of oligonucleotides with ions on an ion exchange resin. <br> <br>4. Affinity chromatography (AC): For the purification of 10-50 nucleotide units in length, selective adsorption and elution of oligonucleotides based on specific interactions between oligonucleotides and paired molecules (e.g. adenine or thymine) on the affinity resin. <br> <br>5. Capillary electrophoresis (CE): Suitable for the purification of oligonucleotides less than 30 nucleotide units in length, the oligonucleotides are separated by electrophoresis. <br> <br>Above are some common methods for oligonucleotide purification, Hanbon can offer relevant equipment, which can be used during the RP-HPLC <br> <br>Choose Hanbon for Your Biopharmaceutical Process <br>In the synthesis stage, we can provide different sizes of synthesizers to meet your needs in different stages of optimization and next step scale-up, from laboratory level to industrial production level; <br> <br>In the purification stage, whether you choose reversed-phase chromatography or ion exchange methods, we have the corresponding equipment for you to choose from; <br> <br>In the ultrafiltration stage, we can also provide different sizes of equipment to meet your needs for salt exchange concentration. <br> <br>In a word, with Hanbon, you can find the system that matches your need for your production process. <br> <br>For more information about simulated moving bed adsorption and simulated moving bed chromatography principle, please feel free to contact us! <br>#simulated#moving#bed#adsorption <br>[a]https%3A%2F%2Fwww.jshanbon.com%2Fproducts%2Fsimulated-moving-bed%2F[/a]

SFC-Dynamic Axial Compression Column (DAC) has the most mature loading technology in the field of preparative chromatography. It can self load, maintain column pressure and self unload, with the function of the chromatographic column and packed column machine. The chromatographic column packed with DAC can meet the requirements of continuity, uniformity, stability and tightness of column bed and eliminate the influence of column-bed collapse. <br> <br>Features of SFC-Dynamic Axial Compression Column <br>01. <br>The DAC column, specially designed for the SFC system, is able to withstand pressures up to 20MPa. This will improve the stability of the system. <br> <br>02. <br>The design of heat preservation jacket of column tube can keep the CO2 in supercritical state when entering the chromatography column, which can meet the reproducibility of the chromatogram. <br> <br>03. <br>The new material of O-type sealing ring can solve the problem of carbon dioxide gas explosion and reduce the failure rate of equipment running for a long time. <br> <br>04. <br>Improved hydraulic principle design with additional &quot;hold&quot; function to prevent CO2 back pressure from causing the piston to move up. <br> <br>For more information about column used in supercritical fluid chromatography are, please feel free to contact us! <br>#column#used#in#supercritical#fluid#chromatography#are <br>[a]https%3A%2F%2Fwww.jshanbon.com%2Fproducts%2Fsfc-dynamic-axial-compression-column%2F[/a]

Hanbon preparative liquid chromatography system has the characteristics of high efficiency, high speed, high automation, and explosion-proof, and is widely used in the separation and purification of pharmaceutical intermediates, biopharmaceuticals, synthetic drugs, natural products, fine chemicals, food additives and other fields. Including: CS-Prep® industrial preparative adsorption hplc system, Newstyle® laboratory liquid chromatography system, Intepure™ Integrated liquid chromatograph etc. <br> <br>The Difference Between Analytical And Preparative Hplc System <br> <br> <br>Analytical chromatography, reflecting the composition of the sample, seeks efficient separation and high sensitivity without the need to collect specific fractions, and eluates are usually disposed of as waste liquids. In preparative process chromatography, however, the maximum amount of product must be produced in the minimum amount of time in order to save investment and operating costs. Due to the difference in purpose, there are major differences in the optimisation of the operating parameters chosen for preparative chromatography and analytical chromatography, including column size, packing, flow rate, operating pressure, injection volume, product purity, product recovery and chromatographic separation effectiveness. The former requires the smaller the injection volume, while the latter requires the column to be overloaded in order to increase the preparation volume. There are two types of column overloading, one is &quot;mass overloading&quot;, where a smaller injection volume is maintained and the injection concentration is increased, and the other is &quot;volume overloading&quot;, where a smaller injection concentration is maintained and the injection volume is increased. In preparative chromatography, mass overloading is generally considered to be preferable. In preparative chromatography separations, both separation and column efficiency decrease as the degree of mass overloading increases. However, operating with mass overload can increase the preparative volume provided that the separation can be achieved. <br> <br>Preferred Options For Impurity Preparation <br>Impurity studies are carried out throughout the life cycle of pharmaceutical development. Impurity preparation is one of the bitter seas of sample purification, and the traditional approach is to use glass chromatography columns or analytical or semi-preparative HPLC to enrich bit by bit. The DAC50 with NS4210 chromatography system is 5-20 times more efficient than a 20 ml/min system with high throughput screening + excellent batch reproducibility. The system can also be equipped with an optional automatic fraction collector to increase automation and allow more focus on drug development. <br> <br>For more information about hplc chromatography steps and latest hplc instruments, please feel free to contact us! <br>#latest#hplc#instruments <br>[a]https%3A%2F%2Fwww.jshanbon.com%2Fproducts%2Fpreparative-liquid-chromatography-system%2F[/a]

Hanbon offers Hanbon preparative HPLC and MPLC from laboratory to industrial level, which are essential tools for the isolation and purification of high-purity small molecules such as intermediates, APIs, insulins, peptides, natural products, etc. <br> <br>What is the difference between preparative and analytical liquid phase? <br>The main difference between preparative and analytical liquids lies not in their appearance but in their purpose. <br> <br>The purpose of the analytical liquid phase is of course to analyse the sample, what it contains and how much it contains, also known as qualitative quantification. To do a good job with analytical liquids, the main concern is separation and sensitivity, to be able to separate, to measure and to be accurate. <br> <br>The preparation of the liquid phase uses the separation power of the column to remove impurities and obtain a high-purity sample. In the laboratory, infrared, ultraviolet and nuclear magnetic instruments all require samples of sufficient purity to give accurate results. In addition to the laboratory, the production of pharmaceuticals also requires purification, especially for biologics and pharmaceuticals. <br> <br>In the laboratory, the liquid phase used for preparation by a preparative liquid chromatography system is configured in much the same way as an ordinary analytical solution. There are pumps, a sample injector, a column and a detector. To collect the separated compounds, the preparative liquid phase is often equipped with a fraction collector. <br> <br>There are many different ways of classifying preparative phases, for example, by pressure or by yield, and there is no clear standard. The different types of preparative phases have different characteristics. <br> <br>What Is Preparative Liquid Phase? <br>The purpose of preparing liquid phase is to extract and purify the monomer of a product. Compared to traditional purification methods (e.g. distillation, extraction), preparative liquid phase is a more efficient separation method and is therefore widely used for the extraction and purification of samples and products. With the increasing demand for high purity components in the synthetic, phytochemical, biochemical and pharmaceutical fields, the field of application of semi preparative chromatography is rapidly expanding and developing. <br> <br>For more information about prep hplc method development and preparative hplc applications, please feel free to contact us! <br>#prep#hplc#method#development

Bio-Oligo synthesizer machine is an efficient, rapid and reliable device for oligonucleotide synthesis independently developed by Jiangsu Hanbon Sci &amp;tech co., ltd. Nowadays, the series is available to customers on two main scales: Bio-Oligo 100 laboratory nucleic automated oligonucleotide synthesis machine and Bio-Oligo Pro 400 pilot scale Bio-Oligo nucleic acid synthesizer machine. To meet different needs of customers from laboratory to pilot scale-up <br> <br>Oligonucleotide From Start to Finish <br>We can provide oligonucleotide synthesis machine system to meet your needs during key areas of oligonucleotide API production, such as synthesis, purification and ultrafiltration. <br> <br>In the synthesis stage, we can provide different sizes of dna oligo synthesizer to meet your needs in different stages of optimization and next step scale-up, from laboratory level to industrial production level; <br> <br>In the purification stage, whether you choose reversed-phase chromatography or ion exchange methods, we have the corresponding equipment for you to choose from; <br> <br>In the ultrafiltration stage, we can also provide different sizes of equipment to meet your needs for salt exchange concentration. <br> <br>In a word, with Hanbon, you can find the system that matches your need for your oligonucleotide production process. <br> <br>Synthesis Principle of Oligonucleotide by Synthesis Machine <br>Most chip oligo synthesis are currently synthesized by solid-phase synthesis, using automated equipment, in which phosphoramidite monomers are required. For example, the phosphoramidite monomer for DNA synthesis mainly consists of bases, deoxyribose, 2-cyanoethyl 5&#039;-DMT and 3&#039;-terminus, and diisopropylamine group, while the phosphoramidite monomer for RNA requires protection of 2&#039;-OH to prevent cross-reaction between 2&#039;-OH and 5&#039;-OH in solid-phase synthesis, and the protecting group is usually TBDMS. Solid-phase synthesis is frequently used nowadays, the traditional solid-phase carriers were controlled microporous glass beads (CPG), but the loading capacity was limited, only 70-80 μmol/g. Nowadays, more high-loading capacity solid-phase carriers are used, and the loading capacity is generally up to 250umol/g~350umol/g. <br> <br>For more information about oligo synthesis price and oligonucleotide synthesizer price, please feel free to contact us! <br>#automated#oligonucleotide#synthesizer <br>[a]https%3A%2F%2Fwww.jshanbon.com%2Fproducts%2Foligo-synthesizer%2F[/a]

Newstyle® portable hplc system is developed by Hanbon Sci&amp;Tech for laboratory analysis, small-scale and pilot scale up. The infusion pump head and liquid circuit contact materials can be selected from SS316L, titanium alloy, PEEK etc., which are suitable for the special requirements of different systems. Dual wavelength UV visible detector, which can expand the detection range, and can also be equipped with differential refractive detector or evaporative luminescence detector. The supporting chromatographic columns include conventional standard liquid chromatography columns, spring columns, dynamic axial compression columns, multifunctional axial compression columns etc. <br> <br>Features of Newstyle® Laboratory portable liquid chromatography System <br>01. <br>The infusion pump adopts electronic damping flow control technology, effectively improving the accuracy of flow rate and gradient; <br> <br>02. <br>Floating piston design, effectively extending the service life of the sealing ring; <br> <br>03. <br>UV visible detector with good detection accuracy and repeatability; <br> <br>04. <br>Dual wavelength detection, expanding the detection range; <br> <br>05. <br>Optional automatic distillate collector, supporting multiple distillate collection methods such as retention time collection, response value collection, and real-time manual collection; <br> <br>06. <br>Rich and completed product specifications can meet the requirements of all stages from development to production. <br> <br>For more information about portable hplc, please feel free to contact us! <br>#portable#hplc#system <br>[a]https%3A%2F%2Fwww.jshanbon.com%2Fproducts%2Fnewstyle-laboratory-liquid-chromatography-system%2F[/a]

Membrane filter holder are package holders for deep filtration, ultrafiltration, and nanofiltration that range in size from 0.1 m² to 20 m². <br> <br>Features of Membrane Cassette Holders <br>01. <br>Adaptations include mainstream brand membrane packs such as millipore / pall / sartorius; <br> <br>02. <br>Special flow path import and export design that guarantees the system is thoroughly cleaned; <br> <br>03. <br>Standard 316L material, with a standard TC interface in the connection port; <br> <br>04. <br>Perfect specifications, from research and development to pilot testing, manufacturing specifications, can achieve custom processing. <br> <br>For more information about tff cassette membrane and membrane filter holder 47mm, please feel free to contact us! <br>#portable#hplc#system <br>[a]https%3A%2F%2Fwww.jshanbon.com%2Fproducts%2Fnewstyle-laboratory-liquid-chromatography-system%2F[/a]

MCC® adopts the compression way by manual screw to load packing materials, with the advantages of concise configuration , easy for cleaning and manual operation, applied to the switching among diversified products. <br> <br>Features of MCC® manual column chromatography <br>01. <br>Strength <br>Imported Schott finished glass tube, easy to seal, high strength. <br> <br>02. <br>Easy to Operate <br>The side sliding design of the column head eliminates the need for manual handling. <br> <br>03. <br>Convenient Replacement <br>The sieve plate can be directly replaced at the bottom without disassembling the column barrel. <br> <br>04. <br>Original Sealing Form <br>Original and highly reliable column head sealing form, manual sealing or automatic sealing. <br> <br>05. <br>Highly Capable <br>Reliable, convenient and safe operation. <br> <br>06. <br>Excellent Performance <br>The flow path has no dead zone design and excellent cleaning and verification performance. <br> <br>07. <br>Certified <br>The liquid receiving material is made of imported materials, which comply with USP Class VI and FDA standards. <br> <br>08. <br>Documents &amp; Services <br>Provide complete GMP verification documents and services. <br>#manual#column#chromatography <br>[a]https%3A%2F%2Fwww.jshanbon.com%2Fproducts%2Fmcc-manual-chromatography-column%2F[/a]