Transcapsidation involves the packing of the ITR from one particular serotype in AAV into the capsids of a different kind of serotype. The majority of cases are based on the extensively studied AAV cell line genome that is wrapped into different AAV serotypes to study the effectiveness. The disadvantage of using transpeptidation for modifying AAV tropism is the possibility of a lower titer, depending on the serotypes employed. Furthermore, due to an interaction of the C-terminus, the coat of protein on the capsid and the genome of the virus results in fewer yields from AAV particles are likely when the virus is produced.