#co2 #chromatography <br>[a]https%3A%2F%2Fwww.jshanbon.com%2Fproducts%2Fsupercritical-fluid-chromatography%2F[/a] <br> <br>SFC (Super Fluid Chromatography) provides precise control over the strength, pressure, and temperature of the mobile phase, enabling fine adjustments to the separation ability and selectivity of the system. This flexibility allows for accurate tuning of the chromatographic conditions to achieve optimal separation and desired analytical outcomes. <br> <br>Chiral Splitting Using SFC (Super Fluid Chromatography) <br>Since the &quot;reaction stop&quot; incident, countries have started to pay attention to the pharmacological activity of isomers in chiral drugs because of the different activities of different isomers. The main chiral dismantling methods are： <br>Crystallization <br>Chemical cleavage <br>Biological dismantling <br> <br>Chromatographic methods, including gas chromatography, for the separation of volatile chiral substances; high performance liquid chromatography (HPLC), which uses a chiral stationary phase with good stability, is an important chiral separation method; supercritical fluid chromatography (SFC), which is environmentally friendly, highly efficient, has a large sample throughput and low cost, has increasingly replaced HPLC as the first choice for chiral separation. <br> <br>The Difference Between SFC and HPLC <br>Preparative high-performance liquid chromatography (Prep HPLC) has been one of the most commonly used purification techniques for over 20 years. Specifically, it is a popular separation process in the fine chemicals, pharmaceutical, and biotechnology industries and is widely used in product purification operations. Over this time, Prep HPLC has developed into a fairly effective and applicable technique, particularly for non-chiral purifications. <br> <br>Although popular, Prep HPLC has several drawbacks. The volume of the mobile phase required to purify a specified mass of the compound is large compared to the total sample volume being processed. A typical Prep HPLC fraction contains a large amount of solvent (organic and aqueous) and because of the time required to dry and collect the final product a major advantage of Prep SFC is that the use of CO2 replaces most of the mobile phase, saving solvent usage. This is a major advantage of Prep SFC, as it uses CO2 to replace most of the mobile phase, saving solvent use and effort. <br> <br>A major advantage of Prep SFC is that it uses CO2 instead of most of the mobile phase, saving solvent usage and making it greener. In SFC, the separation efficiency is better due to the low viscosity and high diffusion rate of the mobile phase, allowing for faster and more efficient chromatographic separations. <br> <br>We provide supercritical fluid chromatography equipment, sfc purification system, sfc mobile phase, supercritical fluid chromatography mass spectrometry, co2 chromatography, supercritical co2 chromatography, mobile phase in supercritical fluid chromatography, etc. For supercritical fluid chromatography makes use of supercritical fluid as or more information, please feel free to contact us!

#single #use #filtration <br>[a]https%3A%2F%2Fwww.jshanbon.com%2Fproducts%2Fsingle-use-tangential-flow-filtration-system%2F[/a] <br> <br>The pump heads, valves, piping and liquid contact parts such as pressure sensors, flow meters, pH, conductivity and UV detectors of single-use UF/DF systems are all made of disposable replaceable parts, all of which are designed to be pre-packaged aseptically in advance and installed directly after unpacking before use, eliminating the need for CIP verification. Particularly suitable for applications where multiple CDMOs or multiple products are in common line. <br> <br>Features of single use tff system <br>01. <br>Direct replacement of any components that come into touch with liquid is quick and easy; <br> <br>02. <br>Give FDA and USP VI certifications for non-stainless steel components coming into contact with liquid; <br> <br>03. <br>Software that is similar to non-disposable systems makes it simple to understand and operate. <br> <br>For more information about single use tangential flow filtration, please feel free to contact us!

#single #use #chromatography <br>[a]https%3A%2F%2Fwww.jshanbon.com%2Fproducts%2Fsingle-use-system%2F[/a] <br> <br>Single use filtration systems specifically designed for the amplification and production of processes in fields such as ADC, mRNA vaccines, plasmid DNA, genes, and cell therapies, utilizing disposable pipelines and flow pools to achieve fast and flexible biological processes. <br> <br>Types of Single-use System <br>Single use systems for biopharmaceuticals <br>Replaceable disposable parts make up the pump heads, valves, tubing, and liquid-contact portions of single-use chromatography systems such pressure transducers, flow meters, pH, conductivity, and UV detectors. <br> <br>Single use filtration System <br>There is no requirement for CIP verification because all parts are made to be aseptically pre-packaged beforehand and inserted right after unpacking before use. especially well-suited to applications where various CDMOs or numerous goods are used in the same line. <br> <br>We provide single use biopharma, single use chromatography, single use technology in biopharmaceutical manufacture, single use biopharma processing, etc. For more information, please feel free to contact us!

#single #use #biopharma <br>[a]https%3A%2F%2Fwww.jshanbon.com%2Fproducts%2Fsingle-use-chromatography-system%2F[/a] <br> <br>The pump heads, valves, tubing and liquid contact components of single use chromatography skid systems such as pressure transducers, flow meters, pH, conductivity and UV detectors are made up of disposable replaceable parts, all of which can be packaged aseptically in advance and installed directly after unpacking prior to use without the need for CIP validation. Suitable for use in virus purification or multiple product co-lines. <br> <br>Features of Single use Chromatography System <br>01. <br>All parts that come into contact with the liquid can be directly replaced, which is fast and convenient; <br> <br>02. <br>Provide FDA and USP VI certificates for non stainless steel materials in contact with the material liquid; <br> <br>03. <br>Using software that is homologous to non disposable systems allows for fast learning and easy use.

Hanbon is a manufacturer of liquid ssmb chromatography series products with a large production scale and a complete range of products in China. <br> <br>The main products include: Biopharm purification equipment, Oligo synthesizer, industrial preparation simulated moving bed smb chromatography systems, supercritical fluid chromatography systems, continuous chromatography systems, laboratory chromatography instruments, etc., and can provide users with integrated solutions and services centered on chromatographic products. <br> <br>Synthesis Principle Of Oligonucleotide <br>Most oligonucleotides are currently synthesized by solid-phase synthesis, using automated equipment, in which phosphoramidite monomers are required. For example, the phosphoramidite monomer for DNA synthesis mainly consists of bases, deoxyribose, 2-cyanoethyl 5&#039;-DMT and 3&#039;-terminus, and diisopropylamine group, while the phosphoramidite monomer for RNA requires protection of 2&#039;-OH to prevent cross-reaction between 2&#039;-OH and 5&#039;-OH in solid-phase synthesis, and the protecting group is usually TBDMS. Solid-phase synthesis is frequently used nowadays, the traditional solid-phase carriers were controlled microporous glass beads (CPG), but the loading capacity was limited, only 70-80 μmol/g. Nowadays, more high loading capacity solid-phase carriers are used, and the loading capacity is generally up to 250umol/g~350umol/g. <br> <br>Purification Method Of Oligonucleotide <br>Oligonucleotides are short-stranded nucleic acids consisting of less than 20 nucleotide units, and purification methods can be selected according to different oligonucleotide lengths, sequences, structures, and purity requirements. The following are some common oligonucleotide purification methods: <br> <br>1. Polyacrylamide gel electrophoresis (PAGE): Suitable for the purification of oligonucleotides with short length (less than 30 nucleotide units). The size difference between oligonucleotides and impurities is separated by PAGE, followed by cleavage and elution of the target oligonucleotides. <br> <br>2. Reversed-phase high-performance liquid chromatography (RP-HPLC): For purification with a length of 10-50 nucleotide units, oligonucleotides are separated from impurities based on polarity differences. <br> <br>3. Ion exchange chromatography (IEX): For purification of 10-100 nucleotide units in length, oligonucleotides and impurities are separated by adsorption and elution of oligonucleotides with ions on an ion exchange resin. <br> <br>4. Affinity chromatography (AC): For the purification of 10-50 nucleotide units in length, selective adsorption and elution of oligonucleotides based on specific interactions between oligonucleotides and paired molecules (e.g. adenine or thymine) on the affinity resin. <br> <br>5. Capillary electrophoresis (CE): Suitable for the purification of oligonucleotides less than 30 nucleotide units in length, the oligonucleotides are separated by electrophoresis. <br> <br>Above are some common methods for oligonucleotide purification, Hanbon can offer relevant equipment, which can be used during the RP-HPLC <br> <br>Choose Hanbon for Your Biopharmaceutical Process <br>In the synthesis stage, we can provide different sizes of synthesizers to meet your needs in different stages of optimization and next step scale-up, from laboratory level to industrial production level; <br> <br>In the purification stage, whether you choose reversed-phase chromatography or ion exchange methods, we have the corresponding equipment for you to choose from; <br> <br>In the ultrafiltration stage, we can also provide different sizes of equipment to meet your needs for salt exchange concentration. <br> <br>In a word, with Hanbon, you can find the system that matches your need for your production process. <br> <br>For more information about simulated moving bed adsorption and simulated moving bed chromatography principle, please feel free to contact us! <br>#simulated#moving#bed#adsorption <br>[a]https%3A%2F%2Fwww.jshanbon.com%2Fproducts%2Fsimulated-moving-bed%2F[/a]

Bio-Oligo synthesizer machine is an efficient, rapid and reliable device for oligonucleotide synthesis independently developed by Jiangsu Hanbon Sci &amp;tech co., ltd. Nowadays, the series is available to customers on two main scales: Bio-Oligo 100 laboratory nucleic automated oligonucleotide synthesis machine and Bio-Oligo Pro 400 pilot scale Bio-Oligo nucleic acid synthesizer machine. To meet different needs of customers from laboratory to pilot scale-up <br> <br>Oligonucleotide From Start to Finish <br>We can provide oligonucleotide synthesis machine system to meet your needs during key areas of oligonucleotide API production, such as synthesis, purification and ultrafiltration. <br> <br>In the synthesis stage, we can provide different sizes of dna oligo synthesizer to meet your needs in different stages of optimization and next step scale-up, from laboratory level to industrial production level; <br> <br>In the purification stage, whether you choose reversed-phase chromatography or ion exchange methods, we have the corresponding equipment for you to choose from; <br> <br>In the ultrafiltration stage, we can also provide different sizes of equipment to meet your needs for salt exchange concentration. <br> <br>In a word, with Hanbon, you can find the system that matches your need for your oligonucleotide production process. <br> <br>Synthesis Principle of Oligonucleotide by Synthesis Machine <br>Most chip oligo synthesis are currently synthesized by solid-phase synthesis, using automated equipment, in which phosphoramidite monomers are required. For example, the phosphoramidite monomer for DNA synthesis mainly consists of bases, deoxyribose, 2-cyanoethyl 5&#039;-DMT and 3&#039;-terminus, and diisopropylamine group, while the phosphoramidite monomer for RNA requires protection of 2&#039;-OH to prevent cross-reaction between 2&#039;-OH and 5&#039;-OH in solid-phase synthesis, and the protecting group is usually TBDMS. Solid-phase synthesis is frequently used nowadays, the traditional solid-phase carriers were controlled microporous glass beads (CPG), but the loading capacity was limited, only 70-80 μmol/g. Nowadays, more high-loading capacity solid-phase carriers are used, and the loading capacity is generally up to 250umol/g~350umol/g. <br> <br>For more information about oligo synthesis price and oligonucleotide synthesizer price, please feel free to contact us! <br>#automated#oligonucleotide#synthesizer <br>[a]https%3A%2F%2Fwww.jshanbon.com%2Fproducts%2Foligo-synthesizer%2F[/a]

Membrane filter holder are package holders for deep filtration, ultrafiltration, and nanofiltration that range in size from 0.1 m² to 20 m². <br> <br>Features of Membrane Cassette Holders <br>01. <br>Adaptations include mainstream brand membrane packs such as millipore / pall / sartorius; <br> <br>02. <br>Special flow path import and export design that guarantees the system is thoroughly cleaned; <br> <br>03. <br>Standard 316L material, with a standard TC interface in the connection port; <br> <br>04. <br>Perfect specifications, from research and development to pilot testing, manufacturing specifications, can achieve custom processing. <br> <br>For more information about tff cassette membrane and membrane filter holder 47mm, please feel free to contact us! <br>#portable#hplc#system <br>[a]https%3A%2F%2Fwww.jshanbon.com%2Fproducts%2Fnewstyle-laboratory-liquid-chromatography-system%2F[/a]